Experimental GI Oncology Lab
Open Positions
Master Student
Background
Metastatic colorectal cancer (mCRC) harboring the BRAF V600E mutation is the most aggressive and deadly subtype of this malignancy. In clinical practice, molecularly targeted combination treatment with cetuximab (anti-EGFR monoclonal antibody) and Encorafenib (BRAF inhibitor) is the approved standard-of-care, based on pivotal results of the phase III BEACON trial. Although Cetuximab plus Encorafenib (CE) has shown promising short-term efficacy, all patients eventually develop acquired resistance, and long-lasting disease control is rare. The molecular mechanisms of acquired resistance to CE are incompletely characterized, and therapeutic strategies to prevent, delay, or overcome resistance are missing.
​
The Project
Using primary cell lines and patient-derived tumor organoids, you will model drug treatment and resistance development in vitro. Through comprehensive molecular assays, you will investigate signaling pathways and molecular alterations that may contribute to driving the insurgence of resistance. Furthermore, you will employ gene-editing techniques to validate potential therapeutic targets implicated in resistance.
​
Techniques
You will gain proficiency in a range of techniques crucial to our research objectives. This includes modeling drug treatment and resistance development, working with patient-derived tumor organoids, and primary cell lines. Additionally, you will engage in gene editing, and utilize molecular assays such as RT-qPCR, PCR, Western blotting, and IF microscopy. Cell-based assays including viability, proliferation, migration, and invasion assays will also be central to your work.
​
Candidate
We seek candidates with a strong interest in cell biology and molecular cancer research. Initiative and a willingness to learn are paramount. While prior experience with mammalian cell culture and standard molecular biology techniques is advantageous, we prioritize candidates with a genuine curiosity for science and translational research.
Laboratory Technician in Translational GI Cancer Research
Your responsibilities
The successful candidate will conduct experimental research with patient-derived organoids, 2D and 3D human cancer cell lines, and blood samples collected from patients. You will perform cell culture work using standard techniques of molecular and cellular biology, including molecular cloning, DNA/RNA extraction and processing, PCR techniques (such as qPCR and ddPCR), processing of blood samples, immunoblotting, and ELISAs. Additionally, you will support other researchers in the lab, conduct independent experiments, and assist with lab organization and the logistics associated with a multi-center prospective biomarker trial.
​
Your profile
-
Apprenticeship as a laboratory technician or a BSc degree in biology, biochemistry, biotechnology, pharmacy, or a related field.
-
Laboratory experience with cell culture techniques (3D experience is an advantage) and standard molecular and cellular biology techniques.
-
Excellent written and verbal communication skills in English.
-
Highly collaborative and motivated team player.
-
Excellent organizational skills for ordering and maintaining lab equipment and supplies.
​
What we offer
We offer varied and interesting work in an inspiring and supportive environment, within a well-organized, modern research facility. Diversity and inclusion are important to us, and this position is funded by an SNSF grant.
​​